The effect of continuous low doses X-ray on the proliferation of pyramidal cell in hippocampus CA1 in prenatal rats / 中华预防医学杂志

<p><b>OBJECTIVE</b>To explore the effect of low doses X-ray on proliferation of hippocampal pyramidal cell in the area of CA1 in prenatal rat and its relevant mechanism.</p><p><b>METHODS</b>A total of 25 pregnant rats were randomly divided into four experimental groups and one control group. The experimental groups, in a duration of consistent 18 days, respectively received different doses as follows: 0.015 mGy/d, 0.03 mGy/d, 0.06 mGy/d and 0.09 mGy/d. The control group received sham radiation. To observe the density and width of hippocampal pyramidal cell in the area of CA1 by HE stained and observe the expression of the ERK1/2 by IHM.</p><p><b>RESULTS</b>(1) Except C group, all other groups presented increment in width of the level of hippocampal pyramidal cell, compared with C group; H group, M group, L1 group and L2 group were higher than that (F value respectively were 8.475, 33.42, 14.395, 44.955; P value respectively were 0.002, 0.048, 0.030, 0.012). But the phenomenon of inhomogeneity in width in H group was observed, at the same time, the density of cell in H group became looser (F = 4.466, P = 0.017). (2) The expression of ERK1/2 in the hippocampus CA1 was seen in cytoplasm of every group, the average optical density of positive ERK1/2 protein significantly increased in L1 group and L2 group, compared with control group respectively (F value respectively were 4.561, 4.103, P value respectively were 0.044, 0.035).</p><p><b>CONCLUSION</b>Low doses X-ray could promote proliferation of hippocampus CA1 cell in prenatal. The reason could be the increment of the ERK1/2 protein induced by X-ray. When the doses reached 0.09 mGy/d, the excesses proliferation phenomenon was observed.</p>

Expression of nm23 and KAI1 and their clinical significance in gallbladder adenocarcinoma / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the expression of two tumor metastasis suppressor genes nm23 and KAI1 in gallbladder adenocarcinoma, and their clinicopathological significance.</p><p><b>METHODS</b>Specimens and clinical data from 31 gallbladder adenocarcinoma patients were collected. Histopathological grading and the expression of nm23 and KAI1 were detected by HE and immunohistochemical staining, respectively. All cases were followed up for at least three years.</p><p><b>RESULTS</b>Immunohistochemical staining showed that the positive rate of nm23 and KAI1 proteins was 71.0% (22/31) and 61.3% (19/31), respectively. The positive expression rates of nm23 and KAI1 proteins in the early stage carcinomas were significantly higher than those in the moderate and advanced stage ones (P exact = 0.0051 and P exact = 0.0084), and both had an negative correlation with clinicopathologic stage (P trend = 0.0047 and P trend = 0.0058). There was a significant difference in the expression of nm23 and KAI1 proteins among well, moderately and poorly differentiated carcinomas (P exact = 0.0328 and P exact = 0.0020). The expression of nm23 and KAI1 was positively correlated with histopathological grade (P trend = 0.0086 and P trend = 0.0006). There was also a significant difference in the expression of nm23 and KAI1 proteins between 17 survival and 14 dead patients (P exact = 0.0038 and P exact = 0.0001 ). A synergistic effect of nm23 and KAI1 protein on the survival was observed , and seemed to be more important than any individual gene alone (P exact = 0.0005).</p><p><b>CONCLUSION</b>The expressions of nm23 and KAI1 proteins are negatively correlated with clinical stage, but positively with histopathological grade in gallbladder adenocarcinoma. These two tumor metastasis suppressor genes may act synergistically to inhibit the tumor metastasis.</p>

Metastatic suppressor Kangai 1 in primary gallbladder carcicoma: Expression and clinical significance / 第二军医大学学报

Objective: To investigate the expression of metastatic suppressor Kangai 1 (KAI1) expression in the primary gallbladder carcinoma and its clinical significance. Methods: The clinical stages of 35 primary gallbladder carcinoma patients were determined according to the TNM criteria of International Union Against Cancer (UICC). Histopathologic changes and the expressions of KAI1 were detected by H-E and immunohistochemical methods, respectively. All the patients were followed up for 3 years. Results: It was found that 1 patient had carcinoma in situ (0 stage), 13 had early carcinoma (I-II stage), and 21 had intermediate or advanced carcinoma (III-IV stage). Histopathologic grading showed that 14 patients had well-differentiated carcinomas, 10 had moderately differentiated ones, and 11 had poorly differentiated ones. Immunohistochemical results revealed that 60.00% (21/35) of the patients were positive of KAI1, with the positive rate being 92.31% (12/13) in early carcinoma (I-II stage) and 42.86% (9/21) in intermediate and advanced carcinoma (III-IV stage), and the former was significantly higher than the latter (Pexact=0.00 476). The positive rate of KAI1 protein was negatively correlated with the clinical staging of patients (Ptrend=0.004) and positively correlated with histopathologic grading (Ptrend=0.001). The positive rate of KAI1 protein in well differentiated carcinoma was 92.86% (13/14), in moderately differentiated carcinoma was 60.00% (6/10), and in poorly differentiated carcinoma was 18.18% (2/11)(Pexact=0.000 475 between the 3 groups). The positive rate of KAI1 protein was 89.47% (17/ 19) in 19 survival patients and 25.00% (4/16) in 16 death cases. Obvious significance of the expression of KAI1 protein was found between the 2 groups (P=0.001). Conclusion: The positive rate of KAI1 is negatively correlated with the clinical staging but positively correlated with the histopathologic grading in patients with primary gallbladder carcinoma. The high expression of KAI1 genes may indicate a better prognosis of gallbladder carcinoma.